linear dna digest solution components Search Results


pmei restriction endonuclease  (New England Biolabs)
96
New England Biolabs pmei restriction endonuclease
Pmei Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmei restriction endonuclease - by Bioz Stars, 2026-03
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eco ri  (TaKaRa)
99
TaKaRa eco ri
Eco Ri, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eco ri - by Bioz Stars, 2026-03
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t4 dna ligase  (New England Biolabs)
99
New England Biolabs t4 dna ligase
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
t4 dna ligase - by Bioz Stars, 2026-03
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psti restriction endonuclease  (Boehringer Mannheim)
90
Boehringer Mannheim psti restriction endonuclease
Psti Restriction Endonuclease, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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psti restriction endonuclease - by Bioz Stars, 2026-03
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digestion enzyme ncoi r0193s  (New England Biolabs)
96
New England Biolabs digestion enzyme ncoi r0193s
Digestion Enzyme Ncoi R0193s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
digestion enzyme ncoi r0193s - by Bioz Stars, 2026-03
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ecori restriction endonuclease  (New England Biolabs)
99
New England Biolabs ecori restriction endonuclease
Ecori Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
ecori restriction endonuclease - by Bioz Stars, 2026-03
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pcr product  (New England Biolabs)
99
New England Biolabs pcr product
Pcr Product, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr product - by Bioz Stars, 2026-03
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exonuclease iii  (New England Biolabs)
96
New England Biolabs exonuclease iii
Mapping of CD8 T-cell epitopes on defined T. parva antigens. The recognition of autologous immortalized skin fibroblasts transiently transfected with full-length, <t>partial-length,</t> <t>exonuclease</t> <t>III-digested,</t> or cloned fragments of cDNA encoding Tp1, Tp4, Tp7, and Tp8 by antigen-specific CD8 T-cell lines (those from animals BV115 [Tp1], BX063 [Tp4 and Tp8], and BW012 [Tp7]) was assessed by an IFN-γ ELISPOT assay. The results of one of two independent experiments are shown. The data are the mean numbers of SFC/104 CD8 T cells for triplicate cultures. The amino acids included in the antigen peptides are indicated.
Exonuclease Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
exonuclease iii - by Bioz Stars, 2026-03
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saci  (New England Biolabs)
96
New England Biolabs saci
Mapping of CD8 T-cell epitopes on defined T. parva antigens. The recognition of autologous immortalized skin fibroblasts transiently transfected with full-length, <t>partial-length,</t> <t>exonuclease</t> <t>III-digested,</t> or cloned fragments of cDNA encoding Tp1, Tp4, Tp7, and Tp8 by antigen-specific CD8 T-cell lines (those from animals BV115 [Tp1], BX063 [Tp4 and Tp8], and BW012 [Tp7]) was assessed by an IFN-γ ELISPOT assay. The results of one of two independent experiments are shown. The data are the mean numbers of SFC/104 CD8 T cells for triplicate cultures. The amino acids included in the antigen peptides are indicated.
Saci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav asred2 plasmid  (TaKaRa)
86
TaKaRa paav asred2 plasmid
Mapping of CD8 T-cell epitopes on defined T. parva antigens. The recognition of autologous immortalized skin fibroblasts transiently transfected with full-length, <t>partial-length,</t> <t>exonuclease</t> <t>III-digested,</t> or cloned fragments of cDNA encoding Tp1, Tp4, Tp7, and Tp8 by antigen-specific CD8 T-cell lines (those from animals BV115 [Tp1], BX063 [Tp4 and Tp8], and BW012 [Tp7]) was assessed by an IFN-γ ELISPOT assay. The results of one of two independent experiments are shown. The data are the mean numbers of SFC/104 CD8 T cells for triplicate cultures. The amino acids included in the antigen peptides are indicated.
Paav Asred2 Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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paav asred2 plasmid - by Bioz Stars, 2026-03
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dna clean concentrator 25 kit  (Zymo Research)
99
Zymo Research dna clean concentrator 25 kit
Mapping of CD8 T-cell epitopes on defined T. parva antigens. The recognition of autologous immortalized skin fibroblasts transiently transfected with full-length, <t>partial-length,</t> <t>exonuclease</t> <t>III-digested,</t> or cloned fragments of cDNA encoding Tp1, Tp4, Tp7, and Tp8 by antigen-specific CD8 T-cell lines (those from animals BV115 [Tp1], BX063 [Tp4 and Tp8], and BW012 [Tp7]) was assessed by an IFN-γ ELISPOT assay. The results of one of two independent experiments are shown. The data are the mean numbers of SFC/104 CD8 T cells for triplicate cultures. The amino acids included in the antigen peptides are indicated.
Dna Clean Concentrator 25 Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna clean concentrator 25 kit - by Bioz Stars, 2026-03
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xbai  (TaKaRa)
97
TaKaRa xbai
Mapping of CD8 T-cell epitopes on defined T. parva antigens. The recognition of autologous immortalized skin fibroblasts transiently transfected with full-length, <t>partial-length,</t> <t>exonuclease</t> <t>III-digested,</t> or cloned fragments of cDNA encoding Tp1, Tp4, Tp7, and Tp8 by antigen-specific CD8 T-cell lines (those from animals BV115 [Tp1], BX063 [Tp4 and Tp8], and BW012 [Tp7]) was assessed by an IFN-γ ELISPOT assay. The results of one of two independent experiments are shown. The data are the mean numbers of SFC/104 CD8 T cells for triplicate cultures. The amino acids included in the antigen peptides are indicated.
Xbai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Mapping of CD8 T-cell epitopes on defined T. parva antigens. The recognition of autologous immortalized skin fibroblasts transiently transfected with full-length, partial-length, exonuclease III-digested, or cloned fragments of cDNA encoding Tp1, Tp4, Tp7, and Tp8 by antigen-specific CD8 T-cell lines (those from animals BV115 [Tp1], BX063 [Tp4 and Tp8], and BW012 [Tp7]) was assessed by an IFN-γ ELISPOT assay. The results of one of two independent experiments are shown. The data are the mean numbers of SFC/104 CD8 T cells for triplicate cultures. The amino acids included in the antigen peptides are indicated.

Journal:

Article Title: Characterization of the Fine Specificity of Bovine CD8 T-Cell Responses to Defined Antigens from the Protozoan Parasite Theileria parva ▿

doi: 10.1128/IAI.01244-07

Figure Lengend Snippet: Mapping of CD8 T-cell epitopes on defined T. parva antigens. The recognition of autologous immortalized skin fibroblasts transiently transfected with full-length, partial-length, exonuclease III-digested, or cloned fragments of cDNA encoding Tp1, Tp4, Tp7, and Tp8 by antigen-specific CD8 T-cell lines (those from animals BV115 [Tp1], BX063 [Tp4 and Tp8], and BW012 [Tp7]) was assessed by an IFN-γ ELISPOT assay. The results of one of two independent experiments are shown. The data are the mean numbers of SFC/104 CD8 T cells for triplicate cultures. The amino acids included in the antigen peptides are indicated.

Article Snippet: The linearized DNA was purified as described above, and 100-ng aliquots were digested with 1 U of exonuclease III (New England BioLabs, Beverly, MA) in 20-μl reaction mixtures at 37°C for various times (from 0 to 30 min).

Techniques: Transfection, Clone Assay, Enzyme-linked Immunospot